HLA-B27 Direct

The EUROArray HLA-B27 Direct detects all currently known HLA-B*27 subtypes. Furthermore, when a reaction is detected, it is indicated whether the subtypes HLA-B*27:06 or HLA-B*27:09 could be present. With the unique direct procedure, the DNA no longer needs to be isolated. The blood sample is treated with two extraction reagents and can then be used directly in the PCR. Data analysis, data interpretation and electronic archiving are fully automated using the EUROArrayScan software. Numerous controls on the EUROArray HLA-B27 verify the correctness of the results. For every reaction it is verified whether DNA was present in the PCR and whether the primers for the amplification were functional, which is particularly relevant when no reaction is detected. All of these controls ensure a reliable test result with just one PCR reaction.