IFA

Indirect immunofluorescence (IFA) remains the gold standard for autoantibody detection in autoimmune diagnostics. Euroimmun leads this field through unmatched standardization of substrate production and incubation. This page provides an in-depth explanation of the two core technologies that make Euroimmun IFA unique: BIOCHIP substrate technology and TITERPLANE® incubation, which together ensure clarity, reproducibility, and confidence in every result.

Why Euroimmun for IFA?

Euroimmun’s IFA system is defined by decades of innovation focused on delivering reliable, high-quality fluorescence:

BIOCHIP Technology: Standardized millimeter-sized substrate fragments for consistent antigen presentation.

BIOCHIP Mosaics: Multiple substrates arranged in one reaction field for multiplex testing.

TITERPLANE® Incubation: Uniform, simultaneous reagent contact for reproducible fluorescence signals.

Flexible Processing: Manual, semi-automated, or fully automated workflows including EUROPattern and UNIQO 160.

Broad Portfolio: Comprehensive autoimmune and infectious disease testing solutions.

BIOCHIP technology

BIOCHIP technology is the foundation of Euroimmun’s standardized IFA system. It ensures consistent substrate quality, high reproducibility, and the flexibility to combine multiple substrates in a single reaction field.

What Is a BIOCHIP?

IOCHIPs are millimeter-sized fragments of high-quality substrates—such as cultured cells, tissue sections, antigen-expressing cells, or purified antigens—that are mounted on specially designed slides. Each BIOCHIP represents a controlled, uniform testing surface that ensures reproducible fluorescence across thousands of reactions.

TITERPLANE® technology

TITERPLANE® is Euroimmun’s unique incubation system designed to ensure precise, uniform reaction conditions across all samples and all BIOCHIPs, supporting brilliant fluorescence and standardized results.

Uniform, simultaneous incubation

In the TITERPLANE® technique:

Diluted samples are pipetted into hydrophilic fields on a glass reagent tray.
The surrounding tray area is hydrophobic, preventing sample spreading or mixing.
Once all samples are applied, slides with BIOCHIPs are placed face-down onto the tray.

This ensures that: